β3 rabbit polyclonal antibody  (Sino Biological)

Journal: Molecules

Article Title: Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation

doi: 10.3390/molecules28093742

Figure Lengend Snippet: Protein expressions in the recipient nerve tissue one month after the siRNA treatment. ( A ) Western blots showing versican V1 expression and S100, PGP9.5, and β3-tubulin distribution in the sham operation, ES1M si-ctrl, and ES1M si-Chsy1 groups. ( B ) Histogram showing the expression of each protein in the sham-op, ES1M si-ctrl, and ES1M si-Chsy1 groups. β-Actin was used as a loading control. Data are present as mean ± standard deviation from three independent experiments. * p < 0.05, compared to the sham operation group. # p < 0.05, compared to the ES1M si-ctrl group.

Article Snippet: After the protein blocking step, the sections were incubated in rabbit polyclonal anti-S100 antibody (1:1000, Taiclone Biotech Corp, Taipei, Taiwan), rabbit polyclonal anti-Chsy1 antibody (1:1000, Origene, Rockville, MD, USA), rabbit polyclonal anti-versicanV 1 antibody (1:500, Invitrogen, ThermoFisher, Waltham, MA, USA), and rabbit polyclonal anti-β3-tubulin antibody (1:1000, Proteintech, Manchester, UK), with the blocking solution for 24 h at 4 °C.

Techniques: Western Blot, Expressing, Control, Standard Deviation

Journal: Molecules

Article Title: Targeting Chondroitin Sulphate Synthase 1 (Chsy1) Promotes Axon Growth Following Neurorrhaphy by Suppressing Versican Accumulation

doi: 10.3390/molecules28093742

Figure Lengend Snippet: Distribution of β3-tubulin and versican V1 in the regenerating nerve tissue after siRNA treatment. The confocal photomicrographs show the distribution of β3-tubulin and versican V1 expression in the regenerating axons. The recipient nerve tissue (distal end of McN) was immunostained with anti-β3-tubulin (green) and anti-versican V1 (red) in 1-/3- month ESN after siRNA injection. After labeling the sections, nuclei DNA were counterstained with Hoechst33342 (blue). There is both β3-tubulin and versican expression in the axons of the sham operation ( A – C ), ES1M si-ctrl ( D – F ), ES1M si-Chsy1 ( G – I ), ES3M si-ctrl ( J – L ), and ES3M si-Chsy1 ( M – O ) groups. Amplified images are shown on the right. Staining of β3-tubulin is shown in the serial sections of the recipient nerve to indicate the location of the regenerating axon (left panel). Triple arrows indicate a cluster of versican V1 accumulation that hindered the regenerating axon path (middle panel). Note that for the ES3M si-ctrl group ( L ); rectangle and yellow dotted line box) and the ES3M si-Chsy1 group ( O ); rectangle and yellow dotted line box), the majority of β3-tubulin-positive nerve fibers were not co-localized with versican V1. Scale bar = 30 μm. Histogram ( P ) shows an average number of sprouting axons in each group 1–3 months after siRNA treatment. Data are present as mean ± SD; # p < 0.05, compared with the ES1M si-ctrl group.

Article Snippet: After the protein blocking step, the sections were incubated in rabbit polyclonal anti-S100 antibody (1:1000, Taiclone Biotech Corp, Taipei, Taiwan), rabbit polyclonal anti-Chsy1 antibody (1:1000, Origene, Rockville, MD, USA), rabbit polyclonal anti-versicanV 1 antibody (1:500, Invitrogen, ThermoFisher, Waltham, MA, USA), and rabbit polyclonal anti-β3-tubulin antibody (1:1000, Proteintech, Manchester, UK), with the blocking solution for 24 h at 4 °C.

Techniques: Expressing, Injection, Labeling, Amplification, Staining